If you go to The Lost Colony Genealogy & DNA Research Group's web site
you will find the name "Tuley" listed as a "Surname of Interest". Most Tooley's from Virginia and North Carolina have at least one ancestor who spelled their name "Tuley".
The Lost Colony Genealogy & DNA Research Group is trying to prove or disprove that the "Lost Colonists" from Roanoke weren't wiped out, but intermarried with friendly Indians.
"Tuley" is a "Surname of Interest" because early (1700's) Tuley's in eastern North Carolina were known to have married women with surnames that were common among Indians living among the colonists.
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